P. aeruginosa secretes a variety of 2-alkyl-4-quinolone (AQ) metabolites that mediate a broad range of virulence functions, including cell-to-cell communication, toxic activity against host cells, and polymicrobial interactions. Luke’s studies are focused on mass spectrometric analysis of Pseudomonas metabolites, and he is co-mentored by Dr. Maureen Kane in the Department of Pharmaceutical Sciences. One of Luke’s projects is aimed at determining the mechanism by which iron regulates AQ production, both during mono-culture and co-culture. He is also analyzing clinical CF samples for the presence of heme and AQ metabolites to determine if their presence correlates with disease exacerbation.
Djapgne L, Panja S, Brewer LK, Gans J, Kane MA, Woodson SA, Oglesby-Sherrouse AG. (2018) The Pseudomonas aeruginosa PrrF1 and PrrF2 small regulatory RNAs (sRNAs) promote 2-alkyl-4-quinolone production through redundant regulation of the antR mRNA. Journal of Bacteriology. 200(10):e00704-17.
Huang W, Brewer LK, Jones JW, Nguyen AT, Marcu A, Wishart DS, Oglesby-Sherrouse AG, Kane MA, Wilks A. (2018) PAMDB: a comprehensive Pseudomonas aeruginosa metabolome database. Nucleic Acids Research 46(D1) D575–D580.
Reinhart AA, Nguyen AT, Brewer LK, Bevere J, Jones JW, Kane MA, Damron FH, Barbier M, and Oglesby-Sherrouse AG. (2017) The Pseudomonas aeruginosa PrrF small RNAs regulate iron homeostasis during acute murine lung infection. Infection and Immunity 85(5).